She said that she would have punched me into next week and called the police. I asked one of my own daughters today what she would have done had I told her, back when she was 14-15 (the age Ivanka appears to be in the pic) to pose with me like that. I’ve got a thousand photographs of me with my daughters – admittedly none posed like that one! – and I can guarantee that in none of those snaps are my children looking at me like Ivanka is looking at Donald. I keep using the gender neutral here, but if you read the piece and subsequent comments for comprehension rather than for ammunition you’d realise that ‘she’, as you insist on referring to them, is in fact a he.įrom the comments by the random stranger, whose ‘nym is Acolyte of Sagan: They analysed the picture pointing out all of the things that they would view as possible ‘tells’ of an abusive relationship based on their experience, and added the proviso that their current judgement may be clouded by previous experience. The random stranger claims experience of working closely with the social services and other child protection agencies in their capacity as an emergency foster carer, a role in which they dealt with many abused children. The ‘rapey parrot’ refers very clearly to the rapey concrete parrot that Trump is perched next to in the picture, the very sinister one that looks ready to pounce on the other concrete parrot. And Iofee, it seems, apologized only after she confected a notapology and then realized it wouldn’t stifle the critics. But this kind of smear is simply out of place for a professional and reputable journalist. It’s fine to report on Trump, investigate him, and, in op-eds, excoriate him. Frankly, that bothers me less than what I see happening to the press. Now Ioffe has apologized, so I’m not sure whether she should lose her job at The Atlantic, though I think many reputable places would have rescinded their offer. And imagine what would have happened if a journalist tweeted that Bill Clinton was “fucking his daughter”! The left-wing press would have bayed for blood. This does not presage an era of responsible journalism. It seems to be the case that when Trump goes low, it’s okay for journalists to go lower. These and the others are all, like Ioffe, professional journalists. Here’s some other specimens from writers: The rest support her, excuse what she said because of Trump’s own “lewd” remarks, excoriate Politico for firing her, and, amazingly say they share her sentiments in that tweet!. But no worries-Ioffe’s landed a job with The Atlantic:Īnd the latest development: 21 journalists have defended Ioffe, and only one even alludes to her tweet being reprehensible. The realization sank in further as she grasped what she had sent out to her 83,500 followers:Īlas, too late: as Snopes reports, Ioffe was fired from Politico. But she couldn’t quite get to a full apology: Shortly thereafter, Ioffe started realizing that maybe she said the wrong thing. Maybe that’s an appropriate tweet for a troll, but not for someone like Ioffe. I deplore Trump, but we gain nothing when journalists accuse him of incest. Now for a professional journalist, that’s a reprehensible thing to say. One of the Twitchy pieces is amusingly called “Finally, the wait for a pro journalist to imply there’s incest in the Trump family is over.” Indeed, for here’s Ioffe’s Fatal Tweet (now deleted but saved for posterity): Many of her pieces, I should add, are thoughtful and good.) I’ve previously taken apart one of her religion-coddling pieces that was published in Foreign Policy. Ioffe was the Russian correspondent for the New Yorker, then a senior editor for The New Republic, and most recently wrote for Politico. Twitchy ( here and here), as well as many other outlets, reported that journalist Julia Ioffe made a rather substantial gaffe on Twitter.
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During library creation, adaptor sequences are appended to both ends of the DNA molecules in a sample. The workflow for these new sequencing technologies proceeds as follows: library creation, library quantification, massively parallel clonal PCR amplification of library molecules, and sequencing. A key advance facilitating higher throughput and lower costs for several of these platforms was migration from the clone-based sample preparation used in Sanger sequencing to the massively parallel clonal PCR amplification of sample molecules on beads (Roche 454 and ABI Solid) or on a surface (Solexa). The digital PCR assay allows absolute quantification of sequencing libraries, eliminates uncertainties associated with the construction and application of standard curves to PCR-based quantification, and with a coefficient of variation close to 10%, is sufficiently precise to enable direct sequencing without titration runs.Ī new generation of sequencing technologies based on sequencing by synthesis and sequencing by ligation are revolutionizing biology, biotechnology, and medicine. This study is the first to definitively demonstrate the successful sequencing of picogram quantities of input DNA on the 454 platform, reducing the sample requirement more than 1000-fold without pre-amplification and the associated bias and reduction in library depth. We successfully sequenced low-nanogram scale bacterial and mammalian DNA samples on the 454 FLX and Solexa DNA sequencing platforms. We demonstrate the use of digital PCR to accurately quantify 454 and Solexa sequencing libraries, enabling the preparation of sequencing libraries from nanogram quantities of input material while eliminating costly and time-consuming titration runs of the sequencer. Second, each library requires a titration sequencing run, thereby increasing the cost and lowering the throughput of sequencing. For many applications, including metagenomics and the sequencing of ancient, forensic, and clinical samples, the quantity of input DNA can be critically limiting. First, large amounts of sample-typically micrograms-are needed for library preparation, thereby limiting the scope of samples which can be sequenced. This requirement has two unfavorable consequences. Yu B (2014) Setting up next-generation sequencing in the medical laboratory.Next-generation DNA sequencing on the 454, Solexa, and SOLiD platforms requires absolute calibration of the number of molecules to be sequenced. Xuan J, Yu Y, Qing T et al (2013) Next-generation sequencing in the clinic: promises and challenges. Williams R, Peisajovich SG, Miller OJ et al (2006) Amplification of complex gene libraries by emulsion PCR. Meyerhans A, Vartanian JP, Wain-Hobson S (1990) DNA recombination during PCR. J Biotechnol 102:117–124ĭressman D, Yan H, Traverso G et al (2003) Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations. Nakano M, Komatsu J, Matsuura S et al (2003) Single-molecule PCR using water-in-oil emulsion. Nat Methods 7:111–118īuermans HP, den Dunnen JT (2014) Next generation sequencing technology: advances and applications. Mamanova L, Coffey AJ, Scott CE et al (2010) Target-enrichment strategies for next-generation sequencing. Gullapalli RR, Desai KV, Santana-Santos L et al (2012) Next generation sequencing in clinical medicine: challenges and lessons for pathology and biomedical informatics. Metzker ML (2010) Sequencing technologies – the next generation. For routine clinical testing, following library generation, we employ the automated Ion OneTouch™ System that includes the Ion OneTouch™ 2 and the Ion OneTouch™ ES instruments for template generation and enrichment of template-positive ISPs, respectively. We describe the methods of preparation and enrichment of template-positive Ion PGM™ Template OT2 200 Ion Sphere™ Particles (ISPs) on the Ion Personal Genome Machine ® (PGM™) System. Here, we discuss the basic principles, advantages, and challenges of applications of emPCR in clinical testing. Ideally, the dilution is to a degree where each droplet contains a single template molecule and functions as a micro-PCR reactor. The basic principle of emPCR is dilution and compartmentalization of template molecules in water droplets in a water-in-oil emulsion. Emulsion PCR (EmPCR) is a commonly employed method for template amplification in multiple NGS-based sequencing platforms. They provide similar functionality as the Google Finance API and you can use them in the same way. However, there are several other exchange rate APIs available to use, like Open Exchange Rates, Currency Layer, or Fixer.io. It’s worth noting that Google Finance API is deprecated and no longer available for use. For example, if you are building an e-commerce website and want to show prices in multiple currencies, you can use the exchange rate to convert the prices to the desired currency. Once you have the exchange rate, you can use it in your application to calculate the equivalent amount in the other currency. You can also specify a date in the past to get the exchange rate for that specific date. For example, if you request the exchange rate for USD to EUR, the API will return the current exchange rate for that pair. The API will return the current exchange rate for the requested currency pair. You can use any programming language that supports HTTP requests to make the request, such as Python, Java, or C#. The API supports multiple formats for the request, including JSON and XML. Next, you will need to make a request to the API using the API key and the desired currency codes. Once you have done this, you will be able to generate an API key that can be used to access the API. To do this, you will need to create a project in the Google Cloud Console and enable the Google Finance API for that project. The API will return the exchange rate and the converted amount.įirst, you will need to get an API key from Google. You will need to pass in the from and to currencies, and the amount you want to convert. The endpoint for getting currency exchange rates is “”. To do this, you will need to make a GET request to the API endpoint. With your API key and project set up, you can now start using the Google API to get currency exchange rates. This will generate an API key that you will use to access the API. Select “API key” as the type of credentials you want to create. To create credentials, navigate to the “Credentials” tab and click on the “Create credentials” button. Once the Google Finance API is enabled, you will need to create credentials to access the API. Once you have found the API, click on the “Enable” button to activate it. To do this, navigate to the “APIs & Services” dashboard, and search for “Google Finance API”. Once you have created a project, you will need to enable the Google Finance API for that project. What you need to access Google API for Currency Exchange Ratesīefore you can use the Google API to get currency foreign exchange rates API, you will need to create a project in the Google Cloud Console. You can then use the returned data in your application or website to display currency exchange rates to your users. Once you have your key, you can use it to request the API in the form of a URL, specifying the currencies you wish to exchange and the date or time range for which you want to retrieve the exchange rate. To use this API, you will need to sign up for a free API key from the Google Cloud Console. One popular method is the Google Finance API, which allows developers to retrieve real-time or historical currency exchange rates. Google provides a variety of APIs that can be used to retrieve currency exchange rates. For the best chance at avoiding THC altogether, buy CBD isolate from a reputable brand. If the product contains enough THC, it can result in a positive test result. Will using CBD balm result in a positive drug test?Īlthough CBD should not appear on a drug test, many CBD products do contain trace amounts of THC. Balms have a thick, heavy consistency that may not work as well for skin conditions. You can apply CBD balm directly to the area you want to treat, like a sore, stiff muscle or joint.įor topically treating a skin condition like acne, eczema, or psoriasis, consider other types of CBD topicals like lotions or creams. If you notice any signs of irritation, such as redness or itchiness, discontinue use of the product.įrequently asked questions What can I use CBD balm for? Wait 24 hours to see how your skin responds. To do a patch test, you’ll simply place a little bit of the balm on the inside of your wrist. Perform a patch test to make sure you don’t have any allergic reactions before using the product on larger areas of your skin. Topical CBD products are unlikely to cause any of the side effects mentioned above, but it’s best to check the ingredients first to make sure you don’t have any allergies to them.ĭon’t use topicals on broken skin. It’s also important to be careful with any new-to-you topical products. CBD may interact with some medications, including those that carry a grapefruit warning. It’s a good idea to talk with a healthcare professional before trying CBD, especially if you’re taking any medications. Though the World Health Organization says CBD is largely believed to be safe, some people may experience side effects, including: Research each potential brand before you buy their product.
GERTRUDE (active 1930s)ĬANNING, MARY BELLA – SEE HOUSTON, MARY G.ĬARTWRIGHT, ENID M.M. – SEE MELLIAR, G.D.F.īURNS, MARGARET ALICE DELISLE (1888-1974)īURROWES, MARGARET ELEANORE BERESFORD (1866-1936)īUSH, PHYLLIS MADELEINE HYLIE (1896-1943)ĬAMERON, SISTER M. – SEE HASTINGS-GRAY, E.M.īOWERLEY, AMELIA MATHILDA – SEE BAUERLE, A.M.īRADEN, DOROTHY KATHLEEN NORAH (1901-2001)īROWNE, FAITH ISABEL KENWORTHY (1882-1973)īURNEY, GLADYS D.F. She has a particular interest in the work of British women artists and in regional arts and crafts.įor a full list of women in the dictionary, please see below.įebuary 8th 2019 | ISBN-13: 978-1-91 | 396 pages | Print and eBook formats will be available.ĪDENEY, ELAINE THERESE – SEE LESSORE, E.T.ĪILESBURY, CAROLINE, COUNTESS OF (d.1803)ĪLDERTON, DOROTHY EILEEN – SEE ANDREWS, D.E.ĪNTRIM, ANGELA CHRISTINA, COUNTESS OF (1911-84)ĪRCHIBALD, PHYLLIS MURIEL COWAN (1880-1947)īAKER, FRANCES CAUTLEY – SEE FARRELL, F.C.īALL, MAUDE MARY & ETHEL (active 1910s-1940s)īANNER, JOSEPHINA – SEE VASCONCELLOS, J. in 2002 awarded by Manchester University. Hons Degree in 1992 at Bolton University, followed by a Ph.D. Sara Gray over a period of eight years, this book is her third to be published. It includes entries for well-known artists such as Barbara Hepworth, Mary Lowndes, and Alice Woodward, alongside influential but forgotten women such as Mary Symonds, Amy Singer, and Catherine Donaldson. This volume is the largest of its kind to document the lives and careers of some British women artists and decorative artists, published in Britain to date, and helps to shed new light on a still-neglected area of British art and design history. Some of these women are known today, some are not, yet all made valuable contributions in areas such as stained glass, metalwork, pottery, woodcarving, illustration, bookbinding and decoration, sculpture, decorative embroidery, decorative jewellery, and illumination. This comprehensive volume presents the biographies of 1,000 women who were active in the British decorative arts over the last few centuries. Amazon Barnes & Noble Amazon UK Free Delivery Worldwide Waterstones Some minus points of the game are that there are not many new features and the feeling is too familiar for those who have played through the original Pokemon Diamond and Pearl versions. In addition, the game also improved the battle system of Pokemon, introducing new changes in the way of attack and defense. New game features include the ability to choose an avatar for your characters and the ability to take pictures with Pokemon using the in-game camera. The game’s sound is also remarkable, with familiar tunes from the Pokemon series and high-quality sound. The game uses beautiful 3D graphics, allowing the player to see the Pokemon and their habitats in greater detail. One of the strong points of the game is the graphics. As with previous Pokemon versions, this game features traditional elements such as choosing one of three starting Pokemon, collecting Pokemon, enhancing skills, and battling other trainers. In the game, players will take on the role of a Pokemon trainer and travel to explore the land of Sinnoh to capture and confront other Pokemon trainers. This is the 15th-anniversary edition of Pokemon Diamond and Pearl, released on November 19, 2021. While Unity has its critics (as any game engine does), it is a versatile and powerful tool with a proven track record on Switch - that developer ILCA chose Unity makes a lot of sense.Pokemon Brilliant Diamond is a role-playing game developed by Game Freak and published by Nintendo for the Nintendo Switch platform. For people who like to tinker with code behind-the-scenes, though, the use of this engine apparently makes ROM edits and tweaks to text and other elements much easier than they might otherwise have been. What does Brilliant Diamond and Shining Pearl's use of Unity mean for the games? Well, for the vast majority of players, very little - you may notice a logo in the credits, but otherwise you likely wouldn't know what engine the game was built in. Switch's support of Unity - a commonly used engine that powers a great many games across all platforms - has been a factor in the huge library of titles the console has accrued since 2017, a great many of which may not have come to Nintendo's platform had the engine not been supported.īrilliant Diamond and Shining Pearl are made with the Unity game engine! This has resulted in a lot of structural changes with how data is stored compared to Sword & Shield's data storage.- Kurt November 9, 2021 One interesting tidbit that has emerged early, though, is the suggestion from various parties on social media that this pair of games has been built using Unity rather than building off the game engine used for Sword and Shield. For spoiler-sensitive players looking forward to these DS remakes, it's a dangerous time on the internet, but rest assured we'll be avoiding any gameplay spoilers around these parts. Spoiler warning: While we'll avoid displaying any information here that might be considered 'spoilerific', we recommend exercising caution when clicking a link that takes you away from the site.Īs is common these days, the next big release on many a gamer's calendar, Pokémon Brilliant Diamond and Shining Pearl, has reportedly leaked ahead of time, with some people having already captured the game's legendary Pocket Monsters. The company's portfolio already includes AI transcription, smart search, call analytics, and AskFred-ChatGPT bot for meetings.įireflies is an AI assistant that helps transcribe, summarize, and analyze meetings. As the future of work unfolds, our goal is to steer thousands of organizations that rely on Fireflies into this new era, fueling human productivity and sparking unparalleled creativity."ĪI Super Summaries joins Fireflies' impressive suite of offerings, reinforcing its position as a leader in AI-powered meeting solutions. GPT-4 comprehends more information and reasons better than previous models. Ramineni affirms, "The world is experiencing rapid evolution, driven by ChatGPT. New users can sign up or start a 7-day free trial to explore these capabilities. The AI Super Summaries feature is now available for Fireflies' Pro and Business tiers, requiring no additional action from existing users. Super Summaries represent the culmination of these insights, meticulously calibrated with generative AI models," explains Krish Ramineni, CEO and Co-Founder of Fireflies.ai. "After generating meeting notes for millions of people, we've gathered valuable feedback and learned how to make our AI assistant more actionable. Finally, Fireflies.ai compiles a comprehensive set of action items for participants to tackle in post-meeting follow-ups. Then, the meeting assistant creates shorthand notes that mimic human-like paraphrasing. Next, the AI generates a detailed meeting outline with chapters and clickable timestamps for easy navigation. Second, the AI notetaker delivers a concise paragraph synopsis. First, Fireflies identifies and extracts key discussion topics from the conversation. AI Super Summaries enable users to effortlessly distill an entire one-hour call into a quick five-minute read, thanks to five essential components. It’s remarkable, and it’s well worth your time. Stardew Valley is just about one of the most relaxing, engaging, charming, and rewarding games on the market, and it was all created through one man. This game created by the wonderful Eric Barone has found success on just about every gaming platform available, has captivated audiences worldwide, and continues to receive regular updates, all free of charge for the player base, which is a rarity in the world of modern gaming. This game is a dungeon crawler, an opportunity for cathartic exploration, a teller of surprisingly dark and poignant stories, and most importantly, a game that is infinitely playable due to a brilliant, addictive gameplay loop. While you may think that this game is a simple Harvest Moon clone, it’s so much more. Let’s kick off proceedings with an indie game that breaks the mold in so many respects.
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